Snag spot-on adenovirus titers & empty/full ratios with Stunner

The Problem 

When working with adenovirus (AdV) vectors it’s a big deal to get fast answers on capsid titer, empty/full ratio, and if things are aggregated. UV/Vis absorbance at 260 nm (A260) is considered the quickest check of AdV titer – but it requires denaturing your AdV, easily making it a 30 minute assay. On top of that, A260 results get thrown off by protein, DNA contamination, or just about anything else that absorbs. Other more technical methods to get titer, like qPCR, are too slow, hands-on or sample-hungry, and none are ready for a quick, easy check of your adenovirus sample.

The Solution 

Stunner’s unique combination of UV/Vis with static and dynamic light scattering (SLS & DLS) gives researchers a full read-out on AdV titer, empty/full ratio, and aggregation – in about a minute using only 2 µL.

The Proof 

In this seminar you’ll learn how Stunner delivers dye-free, standard-free, and hassle-free titers down to 1E9 vp/mL, and checks the size and aggregation of 96 samples in just 1 hour. We’ll look at all the info Stunner delivers, how these can be used for adenoviral vector sample QC, and how adenovirus from a bunch of different vendors stacks up.
 

Presenter: Nelis Denys (Product Manager, Unchained Labs)

Moderator: Kevin Lance PhD (Marketing Manager, Analytical Instruments, Unchained Labs)